"Be Yourself" is the debut single from the second album of the American rock band Audioslave. The song was released in 2005 and topped the Billboard Hot Mainstream Rock Tracks chart for seven weeks and the Hot Modern Rock Tracks chart for four weeks in 2005.

Apart from "Like a Stone", "Be Yourself" is considered the band's most well-known song, and it is still played on radio stations worldwide.

"Be Yourself" has routinely been heard in multiple mediums. The song is the theme music of the 2005 Raw Diva Search which was held by World Wrestling Entertainment (WWE), and it was used as the entrance music of the winner of that contest, Ashley Massaro. WWE once again used the song as one of the official theme songs to Wrestlemania XXVI. "Be Yourself" was also used in the Scrubs season six premiere episode "My Mirror Image". Along with "Your Time Has Come", the track also appeared in the documentary Warren Miller's: Higher Ground. New York Mets outfielder Shawn Green also used the song before his at-bats at games in 2006.

Prior to the vinyl release of the song, Contactmusic.com held a contest in which fans of the band could submit their pictures, over two dozen of which were selected and featured on the 7" vinyl release of the single.